Turkish European Affairs Minister Egemen Bagis has told a news conference that "full membership to the [European] Union is Turkey's only goal, no other goals can be accepted".
Mr Bagis made the comment following the release of the latest EU progress report.
The European Commission urged the EU to continue talks with Turkey but said no progress was achieved in the last year.
It also expressed concern about tension between Turkey and EU-member Cyprus.
Opposition from Cyprus, as well as French and German reluctance to admit the largely Muslim state, are the main obstacle to Turkish membership and a "privileged partnership" has been mooted as an alternative to regular membership.
In an annual report on countries seeking EU membership, the European Commission criticised Turkey, the largest candidate, for not doing enough to normalise relations with Cyprus.
Mr Bagis said the report was out of focus.
In a reference to a recent dispute over gas drilling rights in the eastern Mediterranean, the Commission told Turkey to avoid threats that could further damage ties.
EU Enlargement Commissioner Stefan Fuele said both Brussels and Ankara were frustrated by the lack of progress in Turkey's EU accession.
Just under half of Turkey's exports are sold to the European Union.
