Taoiseach Micheál Martin has said Dara Calleary has resigned as deputy leader of Fianna Fáil and as national secretary of the party.
The Mayo TD initially apologised on Thursday for attending the event with more than 80 people last Wednesday.
On Friday morning, Mr Calleary tendered his resignation after just 37 days in the ministerial role.
There was further speculation over the weekend about his positions within Fianna Fáil.
Speaking on RTÉ's Today with Claire Byrne this morning, the Taoiseach said that Mr Calleary "knows he got it wrong".
Mr Martin added: "He understands that by going down to speak [in Clifden] he was undermining the authority of Government to bring in restrictions and make it clear about the necessity of these restrictions."
The Taoiseach also said he did not believe that new Supreme Court judge Seamus Woulfe should have been at the event in Clifden.
He said that the judiciary needs to look at his position in light of his attendance at the Oireachtas Golf Society event.
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