Dublin Bus services ceased at 9pm tonight as the latest 48-hour strike by drivers begins in the morning.
Nitelink services will not be in operation for the duration of the weekend as a result of the industrial action.
Normal service will resume on Sunday.
Further strike action is planned for next Tuesday, Wednesday and Saturday.
Drivers have announced a series of strikes in a dispute over pay, with tomorrow and Saturday the latest dates, following four strikes earlier this month.
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Dublin Bus has urged staff to attend the Workplace Relations Commission for negotiations but stressed that it cannot exceed the three-year 8.25% pay deal, with no productivity, awarded by the Labour Court.
However, it is prepared to discuss further increases based on productivity.
Unions are demanding a basic increase of 15% over three years before they will discuss productivity, and object to what they call preconditions.
Dublin Bus Chief Executive Ray Coyne has said the dispute is undermining all past efforts at recovery, and has the potential to return the company to a post-2010 financial crisis.
Mr Coyne says the four strike days to date have cost the company over €4m.
The National Bus and Railworkers' Union has warned that the ongoing industrial action at Dublin Bus may spread to its sister companies at Bus Éireann and Iarnród Éireann.
Minister for Finance Michael Noonan has said the ongoing Dublin Bus strikes are a huge inconvenience for commuters.
He said customers had coped well so far and the weather during previous strike days had been fine but that if that changed it would be a different story.
He would like, he said, if unions and managements used direct talks and all the industrial relations resources that are available in Ireland to resolve the situation.
Minister for Transport Shane Ross yesterday said he would not "ride in on a white horse in shining armour" with a chequebook to solve the dispute.
