Italy midfielder Riccardo Montolivo missed out on his second straight major tournament due to injury while veteran Daniele De Rossi has been included in their 23-man squad for Euro 2016.
AC Milan captain Montolivo, denied a place at the World Cup in Brazil two years ago after breaking his leg in a friendly, suffered a calf injury last week.
De Rossi, 32, has played only four matches for Italy during coach Antonio Conte's two seasons in charge but injuries to Marco Verratti and Claudio Marchisio have also depleted the new Chelsea manager's options in the centre of the park.
West Ham defender Angelo Ogbonna and Manchester United full-back Matteo Darmian are included, while Southampton's Graziano Pelle is one of six forwards in Conte's panel.
Napoli's Brazilian-born midfielder Jorginho was among seven players included in the provisional squad last week who failed to make the cut.
Italy face Ireland in their final Group E match on 22 June, while they also face Belgium and Sweden in the group stage at the finals in France.
Italy squad:
Goalkeepers: Gianluigi Buffon, Federico Marchetti, Salvatore Sirigu
Defenders: Andrea Barzagli, Leonardo Bonucci, Giorgio Chiellini, Angelo Ogbonna, Federico Bernardeschi, Matteo Darmian, Mattia De Sciglio
Midfielders: Antonio Candreva, Daniele De Rossi, Alessandro Florenzi, Emanuele Giaccherini, Thiago Motta, Marco Parolo, Stefano Sturaro;
Forwards: Eder, Ciro Immobile, Lorenzo Insigne, Graziano Pelle, Simone Zaza, Stephan El Shaarawy
