The US House of Representatives is expected to vote on a resolution on Tuesday reaffirming support for the Good Friday Agreement.
The bill was unanimously passed by the House Foreign Affairs Committee in October.
The bipartisan resolution is co-sponsored by the Democratic Congressman, Thomas Suozzi, and Republican Congressman, Peter King.
It calls for strict adherence to the Good Friday Agreement during Brexit negotiations.
The legislation urges the European Union and the United Kingdom to ensure that Brexit does not threaten peace on the island of Ireland. It strongly opposes the reintroduction of a hard border.
It also insists that any trade agreements between the United States and the UK are contingent on meeting the Good Friday Agreement's obligations.
A number of senior members of Congress have warned that they will oppose any US-UK trade deal that undermines the Good Friday Agreement.
Those who have voiced their opposition include the House Speaker Nancy Pelosi, Senate Minority Leader Chuck Schumer, Chairman of the House Ways and Means Committee Congressman Richard Neal, and Democratic Congressman, Brendan Boyle.
Last month, the former US vice-president Joe Biden voiced his support for protecting the Good Friday Agreement and avoiding a return to a hard border after Brexit.
Mr Biden, who is seeking the Democratic Party nomination for president, said he would continue to put pressure on the UK and that he has made it clear in the past that he does not support "breaking down" what is currently in place.