The Bill allowing pubs to sell alcohol on Good Friday has been signed into law by President Michael D Higgins.
Last week the Dáil passed the Intoxicating Liquor (Amendment) Bill 2017.
Introducing the bill, Minister of State David Stanton said removing the ban on selling alcohol on Good Friday would help Irish tourism.
The provision banning the sale of alcohol on Good Friday was introduced in 1927.
That piece of legislation also prohibited the sale of alcohol on St Patrick's Day and Christmas Day.
While the Christmas Day prohibition remains in place, retailers have been allowed to sell alcohol on St Patrick's Day since the 1960s.
Time called on Good Friday alcohol ban