Tusla has confirmed that the Garda Child Protection Unit at Mountjoy Garda Station is opening an investigation, along with the Tusla Social Work Unit, into the issues raised by RTÉ Investigates into standards of care at the Hyde & Seek Childcare chain.
If parents believe that their children are affected, they can contact gardaí at Mountjoy on 01 666 8600.
Earlier, Hyde & Seek issued a statement saying it will bring in external experts to review its management structure.
Hyde & Seek said the RTÉ Investigates documentary, broadcast last night, "raised some real issues for us and we will deal with them quickly".
It said the overall picture painted "does not reflect who we are" and it disputes some of the detail of what was reported.
But, it added, there are specific issues "we need to address and are addressing quickly".
RTÉ Investigates: Crèches, Behind Closed Doors went undercover to look at standards of care in the company.
RTÉ Investigates was contacted by several families who were concerned about the standards of care their children had received while at various Hyde & Seek Childcare crèches.
Two undercover researchers were employed by the company, which is a family-run business owned and run by Anne and Peter Davy and their daughter Siobhan Davy.
The company has four crèches across Dublin city catering for children from three months up to 12 years old.
Anne Davy is to step down and take no future role in frontline childcare provision.
In the statement released today, Hyde & Seek said it has begun to identify an expert consultant this week and one of the first changes being made is the recruitment of a new manager at its Tolka Road crèche.
The statement continued: "We know we need to work to rebuild, retain and enhance the trust our parents have in us. We have spoken to many of them in recent days and would urge others with concerns to contact us.
"We are available to talk to and meet parents at any time.
"We note that while the programme made criticisms of aspects of our service, it praised our childcare staff whose dedication, professionalism and kindness are central to the care we provide".
Parents with concerns about any provider can contact the child and family agency Tusla.