The Garda Representative Association has voted unanimously reject the Garda Reserve.
The GRA’s outgoing President, Dermot O'Donnell, said rank and file gardaí would now refuse to cooperate with the reservists.
Earlier, the General Secretary of the GRA, PJ Stone, overruled his association's newly-elected president and has rejected the targeting of politicians or marginal constituencies as part of its campaign against the reserve.
Mr Stone said gardaí wanted to be involved in policing, not politics, and he said if the impression was given otherwise, he was very sorry.
Referring to the comments by John Egan yesterday, Mr Stone said sometimes people go further than they should and make mistakes and that was regrettable.
He said he hoped the original statement would not damage the association. However, he insisted that the GRA was still totally opposed to the Garda Reserve and would not cooperate with it.