Trent Alexander-Arnold will miss Euro 2020 after the Liverpool right back withdrew from the England squad with a thigh injury.
He sustained the injury during the closing stages of England's 1-0 friendly win over Austria on Wednesday and British media reported he faces a recovery period of four to six weeks.
"A scan on Thursday confirmed the 22-year-old will be unable to play in the forthcoming tournament so he's withdrawn from the England squad to return to his club for rehabilitation," England said in a statement.
England manager Gareth Southgate "will not confirm a replacement until after Sunday's return to the Riverside Stadium for the warm-up fixture against Romania".
Alexander-Arnold was dropped from the England squad in March after Southgate said he had suffered a dip in form, but he was reinstated after helping Liverpool to a third-placed finish in the Premier League.
The 22-year-old was included in England's final Euro squad with fellow right backs Kieran Trippier, Kyle Walker and Reece James.
Southgate is likely to call up an additional player from one of Jesse Lingard, James Ward-Prowse, Ollie Watkins, Aaron Ramsdale, Ben Godfrey and Ben White, who were all cut from England's squad on Tuesday.
England start their campaign against Croatia in the Group D opener on 13 June before they face Scotland on 18 June and the Czech Republic on 22 June, with all the games being played at Wembley.