England midfielder Michael Carrick sustained an ankle injury during England's 2-0 friendly defeat in Spain on Friday.
The 34-year-old Manchester United player was carried off the pitch on a stretcher late in the game.
"Michael has got a severely twisted ankle which normally means ligament damage," England manager Roy Hodgson told reporters.
"We won't know that of course until he's had an X-ray or a scan, but it doesn't look good."
Carrick is definitely ruled out of Tuesday's friendly against France at Wembley Stadium, which goes ahead despite the terror attacks in Paris on Friday night.
