Scotland omitted Finn Russell from their squad to face England as they look to maintain "cohesion" ahead of their second Guinness Six Nations game.
But head coach Gregor Townsend remains in talks with the sidelined Racing 92 fly-half.
The Racing 92 fly-half missed Scotland's 19-12 defeat to Ireland at Dublin on Saturday after being disciplined for breaching team rules last week following an alleged late-night drinking session at the Dark Blues' team hotel.
Adam Hastings is set to continue in the number 10 jersey for Saturday's BT Murrayfield encounter, while Duncan Weir is also in the 37-man squad.
Meanwhile, they will again be without wing Darcy Graham, who remains sidelined due to a knee injury.
But it's 27-year-old playmaker Russell's continued absence from head coach Gregor Townsend's latest group that will inevitably raise further questions about whether he will be involved at all in this season's tournament.
Defence coach Steve Tandy said: "I think Gregor is in dialogue with Finn but I know through this period we have been after cohesion and as many players to do as many training sessions as we can."
When asked whether Russell would feature later in the tournament, Tandy said: "I have no idea. That's as much as I know and as defence coach I think I have enough to deal with. It's more about getting our systems up and running. I will leave that to the boss."
Lock Jonny Gray hopes to see his former Glasgow team-mate back in the squad.
"I don't know too much about the situation," Gray said. "All I know is that everyone cares about Finn here and hopefully it sorts itself out."
Gray was still hurting from Saturday's 19-12 defeat after Scotland failed to make the most of a series of chances, the most blatant when captain Stuart Hogg spilled the ball while attempting to touch down.
"It's still pretty raw," the 25-year-old said. "All credit to Ireland, they ground out the win and deserved it, but it's frustrating from our end not to take our opportunities. It hurts to lose.
"It was tough watching the review, just how gutted we were that we didn't take those opportunities. There were a lot of positives but we know we have to do better in those areas, and conceding penalties and turnovers as well."
Speaking on the RTÉ Rugby Podcast prior to Ireland and Scotland's opener, BBC Scotland chief sports writer Tom English discussed the relationship between Russell and Townsend.
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"Russell thinks that Townsend is overly hard on him," he said.
"Townsend would say that that's nonsense. But certainly Russell has felt under pressure.
"They had a two or three hour meeting and Townsend thought that everything had been resolved.
"They spoke at length at a pretty deep level and the day after Russell announced that he was heading back to France and that really surprised Townsend because he thought the clear the air chat had worked.
"Clearly it hadn't."
In Russell's absence on Saturday, Scotland put Ireland under pressure but were unable to prise open the home defence for a try as they just fell short.
Scotland's 37-man squad for Round Two
Forwards: Simon Berghan (Edinburgh), Jamie Bhatti (Edinburgh), Magnus Bradbury (Edinburgh), Fraser Brown (Glasgow Warriors), Alex Craig (Gloucester), Luke Crosbie (Edinburgh), Scott Cummings (Glasgow Warriors), Allan Dell (London Irish), Cornell du Preez (Worcester Warriors), Zander Fagerson (Glasgow Warriors), Grant Gilchrist (Edinburgh), Tom Gordon (Glasgow Warriors), Jonny Gray (Glasgow Warriors), Nick Haining (Edinburgh), Stuart McInally (Edinburgh), Willem Nel (Edinburgh), Jamie Ritchie (Edinburgh), Rory Sutherland (Edinburgh), Ben Toolis (Edinburgh), George Turner (Glasgow Warriors), Hamish Watson (Edinburgh)
Backs: Chris Harris (Gloucester), Adam Hastings (Glasgow Warriors), Stuart Hogg (Exeter Chiefs), George Horne (Glasgow Warriors), Rory Hutchinson (Northampton Saints), Sam Johnson (Glasgow Warriors), Huw Jones (Glasgow Warriors), Blair Kinghorn (Edinburgh), Sean Maitland (Saracens), Byron McGuigan (Sale Sharks), Ali Price (Glasgow Warriors), Henry Pyrgos (Edinburgh), Matt Scott (Edinburgh), Kyle Steyn (Glasgow Warriors), Ratu Tagive (Glasgow Warriors), Duncan Weir (Worcester Warriors)