Harry Kane has told those who racially targeted some of England's players after the Euro 2020 final defeat to Italy "we don’t want you" while boss Gareth Southgate described the abuse as "unforgivable".
Marcus Rashford, Jadon Sancho and Bukayo Saka were all subjected to abusive posts in the wake of their missed penalties in the shoot-out.
Kane said on Twitter: "Three lads who were brilliant all summer had the courage to step up and take a pen when the stakes were high.
"They deserve support and backing not the vile racist abuse they’ve had since last night. If you abuse anyone on social media you’re not an England fan and we don’t want you."
England manager Southgate said at a press conference on Monday morning: "For some of them to be abused is unforgivable really.
"I know a lot of that has come from abroad, that people who track those things have been able to explain that, but not all of it.
"It’s just not what we stand for. We have been a beacon of light in bringing people together in people being able to relate to the national team, and the national team stands for everybody and so that togetherness has to continue.
"We have shown the power our country has when it does come together and has that energy and positivity together.
"We heal together as a team now, and we’re there for them, and I know that 99 per cent of the public will be as well."
Midfielder Jude Bellingham, who made three substitute appearances in the tournament but did not feature in the final, labelled the abuse "hurtful but not surprising" and insisted he will "never get bored of saying that more needs to be done".
Defender Reece James posted: "We learn more about the society when we lose, far more than we learn when we win."
Arsenal released a statement stating their pride in how Saka had represented his club and his country during the tournament, which had turned to sorrow when witnessing the abuse the 19-year-old suffered.
"We are sad to have to say we condemn the racism of a number of black players," the statement read.
"Our message to Bukayo is: hold your head high, we are so very proud of you and we cannot wait to welcome you back home to Arsenal soon."
Manchester United compiled messages of support for striker Rashford on their website, and a statement introducing the posts said: "It takes tremendous guts to take a penalty under such pressure, when the entire nation is anxiously watching on, so we are proud that Marcus was brave enough to step up."
Former England and Manchester United team-mate Rio Ferdinand highlighted that the players who missed would have been idolised by those who abused them had the result gone the other way.
He tweeted: "These same idiots only days ago would have been celebrating the brilliance of a (Raheem) Sterling or a Saka or a (Kyle) Walker for their efforts in an England shirt. You can bet ya last pound also that they would have sung the Raheem Sterling song almost bursting the veins in their bodies doing so.
"But England lose and that joy and sense of being united immediately evaporates and our black players become the target."
Twitter said it had proactively removed more than 1,000 posts over the past 24 hours which violated its policy and also taken swift action to permanently suspend a number of accounts.
Facebook, which owns Instagram, said it tries to remove harmful content as quickly as possible and encouraged people to use the tools it offers to block abuse.
Meanwhile, the UK Football Policing Unit (UKFPU) has also launched an investigation into the racist hate targeted at players following the match.
Chief Constable Mark Roberts, National Police Chiefs’ Council Football Policing Lead, said: "Investigations are under way to identify the offenders.
"We have had excellent support during the tournament from Facebook, Instagram and Twitter and enquiries are already being progressed.
"When an offender’s details are identified, this is passed onto the relevant local force to investigate further and take appropriate action in conjunction with the CPS. The UKFPU is also working closely with the FA, ensuring the relevant support is in place where needed."