The first sailing from Holyhead Port since its temporary closure in early December is scheduled to depart at 1.30am on Thursday morning.
The port was closed after damage was caused to the ferry terminals during Storm Darragh.
A spokesperson for the port said that it plans to operate "a full schedule for both ferry operators [Irish Ferries and Stena Line] from Terminal 5 on a temporary basis".
The spokesperson added that the port, which is operated by Stena Line, will provide an "update" on a timeline for the resumption of services at Terminal 3 "as soon as possible".
Minister of State at the Department of Transport James Lawless thanked "haulage and associated sectors for the unbelievable effort they put in over the holiday period, going above and beyond the call of duty to ensure Christmas was saved for so many families and businesses".
President of the Irish Road Haulage Association, Ger Hyland said he was delighted to see the port reopening, adding that "it would alleviate a major problem for Irish hauliers".
However, he expressed concern that there was no definite timeline for the reopening of Terminal 3, explaining that the industry would be "back to square one" should Terminal 5 close again due to adverse weather.
The impact on the local economy in north Wales has been significant.
As a result, the Irish, Welsh and UK governments have agreed to set up a taskforce involving key players in the ports and ferry industry to help achieve the "long term resilience" of Holyhead Port.
