Austrian rail operator OeBB has said that it has filed complaints against two people after they had allegedly manipulated the trains' loudspeaker announcements to play what appeared to be Hitler salutes and speeches.
The incident comes after two trains had been manipulated last week to broadcast a "nonsensical, confusing mix" of childrens' songs and old, flawed announcements, OeBB spokesman Bernhard Rieder saood.
The latest incident, which provoked public outcry, allegedly included Hitler salutes and what appeared to be historic recordings of speeches by Adolf Hitler.
All three incidents occurred when OeBB trains passed through the same section between the eastern Austrian city of St Poelten and its capital Vienna, Mr Rieder added.
Yesterday evening, the two suspects were reported to Austrian police, who identified them through analysing video footage from their trains' surveillance cameras.
The OeBB declined to further identify the suspects, but confirmed the two are "not OeBB employees".
The suspects are accused of allegedly obtaining a generic key common throughout Europe to gain access to the train's intercom station to play back the audio recordings, Mr Rieder added.
Adolf Hitler was born in Austria.
Austria - which the Nazis "annexed" into the Third Reich in 1938 - has some of the world's strictest laws against Holocaust denial and pro-Nazi activities.
Despite this, offences involving expressions of pro-Nazi sentiment are not uncommon.