Al-Qaeda chief Ayman al-Zawahiri has rejected the US apology over the burning of Koran copies at a base in Afghanistan, urging all Muslims to support the Taliban.
"The Crusaders once again repeated their crime by insulting the holy Koran, and once again mocked the messenger of Allah," Mr Zawahiri said in a SITE English-language translation.
In February, thousands of Afghan protesters attacked the biggest US military base in their country, at Bagram near Kabul, reacting to reports that troops inside had burned copies of the Koran.
Around 40 people were killed in several days of violent protests.
American officials say the Korans had been confiscated from prisoners as they used them to communicate between each other.
The incident led US President Barack Obama to apologise for what he described as an error.
In today's video which the US-based SITE said was posted on extremist forums, Mr Zawahiri criticised Obama's apology.
"After each of their crimes, they pretend to be sorry, and they claim they will investigate what happened, which is a silly farce that Obama and his secretary repeated this time also," said the chief of the terror network.
"The American Crusaders and their allies showed over and over again their hatred and envy of Islam, the book of Islam, the prophet (of) Islam," Mr Zawahiri said.
He urged Muslim across the world to "fight the enemies of Allah and the enemies of His Messenger."
Mr Zawahiri delivered a similar message in March, urging Afghans to rise up against "Crusader pigs" after US Marines were shown in an Internet video urinating on the corpses of Taliban militants.
A fresh scandal has rocked the alliance between the United States and the Afghan government every month this year in their joint efforts against Taliban insurgents.
A US soldier in March went on the rampage and murdered 17 Afghan villagers in their homes.
