Actor Kevin Spacey pleaded not guilty to indecently assaulting a teenager who alleged that the Oscar-winning US actor groped him in a bar in Massachusetts in 2016.
The former "House of Cards" star, who faces five years in prison if convicted, is accused of sexually assaulting William Little in 2016.
Mr Little was 18 at the time of the alleged assault at a Nantucket restaurant.
Mr Spacey was not asked for his plea during the brief arraignment in a crowded Nantucket courtroom, but his lawyer entered the denial on his behalf.
A follow-on hearing was scheduled for 4 March, but Mr Spacey is not required to attend.
The 59-year-old actor stood quietly with his hands clasped as the clerk read out the charge "that in the town of Nantucket on July 18, 2016 (the defendant) did commit indecent assault and battery on someone 14 and over."
The case marks the first time Mr Spacey has faced sexual misconduct charges, although there are dozens of claims against him.
They come more than 13 months after the complaint was filed, the latest chapter in Hollywood's reckoning over sexual assault and harassment that has already seen television icon Bill Cosby jailed and movie mogul Harvey Weinstein indicted.
Mr Spacey's alleged victim was working as a bus boy for the summer in Nantucket and had remained in the bar after his shift finished to see Mr Spacey, of whom he was a fan.
After meeting the actor and telling him he was 23-years-old - the legal drinking age is 21 in Massachusetts - he said he started drinking first beer and then whiskey at Mr Spacey's urging.
The actor then allegedly invited him back to his place, along with some other friends.
The young man declined, suspecting the actor was trying to seduce him.
But he stayed at the bar in the hopes of getting a picture with Mr Spacey.
It was then that the actor began sexually molesting the young man, according to court filings.
The two-time Oscar winner was considered one of the finest actors of his generation.
His career nosedived following allegations of sexual misconduct by more than a dozen men in the United States and Britain.
Forced out of public life, Mr Spacey posted a video online last month in which he discussed allegations of sexual abuse in character as Frank Underwood, the scheming politician he played in "House of Cards" before the show dropped him.
"You wouldn't believe the worst without evidence, would you? You wouldn't rush to judgments without facts, would you? Did you? No, not you. You're smarter than that," Mr Spacey said in the video.
"Of course, some believed everything and have been just waiting with bated breath to hear me confess it all. They're just dying to have me declare that everything said is true and that I got what I deserved.
"Only you and I both know it's never that simple, not in politics and not in life."
In addition to the Nantucket case, two other investigations are underway in Los Angeles and London, where Mr Spacey served as artistic director of the prestigious Old Vic theater from 2004 to 2015.
Mexican actor Roberto Cavazos, who performed in several Old Vic productions, said it seemed that "it only took a male under 30 to make Mr Spacey feel free to touch us."
The first public allegation of abuse came from actor Anthony Rapp, who claimed that Mr Spacey sexually assaulted him when Rapp was 14, in 1986.
Mr Spacey has not appeared on the screen or on stage since Mr Rapp came forward.
Director Ridley Scott even removed Mr Spacey from his film "All the Money in the World" by shooting all scenes in which the actor appeared again with another star, Christopher Plummer.
