The Dáil was suspended for a time during a debate on a bill brought forward by Sinn Féin that seeks to prevent the Central Bank from dealing with Israeli bonds.
The five-minute interruption happened after around 40 Palestinian demonstrators began to chant in the public gallery.
Several visitors were removed as a result of the disruption.
The protesters reacted after Minister for Finance Paschal Donohoe detailed why the Government was opposing the private members' motion.
"I hope the Dáil can appreciate this evening that any attempts by the Government to introduce measures outside the agreed legal framework in the area pose a significant policy risk," Mr Donohoe said.
In her opening comments, Sinn Féin leader Mary Lou McDonald said it was "shameful that the Irish State and Government would have any hand, act or part in supporting Israel's genocide".
However, Minister of State Marian Harkin said that "any attempt [by Ireland] to act unilaterally on matters as sensitive and complex as financial restrictions could undermine the coalition we are trying to see form at EU level".
"Ireland has been painstakingly involved in helping to build this coalition, and in its context I think we have to prioritise coordinated action through the EU."
In his closing remarks, Sinn Féin's finance spokesperson Pearse Doherty accused Mr Donohoe of delivering a speech that was "full of nonsense" and would come back to bite him.
"What this legislation does is it prevents our Central Bank from approving the [Israeli bond] prospectus ... It would be up to Israel to go and apply to another home country which wanted to do that," the Donegal TD said.
"We got a legal opinion from them [The Office of Parliamentary Legal Advisers] which said this legislation was constitutionally compliant, compliant with EU law, and according with international law."
Strongly criticising the Government for not allowing the bill go to committee stage, he appealed to other deputies: "Stand on the right side of history and support this legislation".
The debate then concluded with a vote to take place tomorrow night.
