Garda Commissioner Drew Harris has said he is going to write to the Ceann Comhairle about allegations made against gardaí under Dáil privilege which he said were "inaccurate".
People Before Profit-Solidarity TD Richard Boyd Barrett told the Dáil that Mothers Against Genocide protesters were "arrested very, very violently in some cases" and repeated an allegation by a woman that she had been "stripped completely naked" in garda custody.
His colleague Paul Murphy said the women had been subjected to "absolutely horrific" treatment.
Mr Harris said "immediately" the allegations were made, a preliminary inquiry commenced.
He said today that he can "categorically state" that "no cavity search took place" and that "no strip search took place".
The Commissioner said that the two gardaí who dealt with the "arrested individual" followed procedures.
He said he had spoken to them and they provided "a full account", there was body-worn footage from the Dáil, and footage from within the station and the custody area.
Mr Harris said he was "entirely content" that "there was no wrongdoing" and the two gardaí have his "full 100% support".
Mr O'Callaghan said at the Association of Garda Sergeants & Inspectors conference last night that he contacted Commissioner Harris on foot of the allegations.
Commissioner Harris said he had conducted inquiries and reported back to Mr O'Callaghan last week "in writing" that the allegations were false.
He also said the allegations were very upsetting and damaging for the female gardaí accused of this "grave wrongdoing".
Mr O'Callaghan said that politicians should reflect on that before standing up in the Dáil to make very serious allegations about identifiable gardaí.
He also said they should inform people making allegations that the appropriate forum to make such complaints is to go to the statutory agency responsible for those complaints, Fiosrú, which is the new body established last week.
"Any such investigation conducted by Fiosrú will also have access to the recordings examined by the Commissioner," he said.
They were "arrested very, very violently in some cases" despite being on a peaceful protest, he said.