Around 1,500 people are expected to attend the Velo City event at the National Convention Centre.
The event, organised by the European Cycling Federation and hosted by Dublin City Council, is the biggest cycling conference in the world.
Topics being discussed include how cyclists and autonomous vehicles will interact, how cargo bikes can replace lorries or vans for city centre deliveries and cycling and climate change.
The theme of this year's conference is Cycling for the Ages, which the organisers say reflects the focus on the future for cycling in cities and also the need to make spaces where people of all ages can cycle.
There will also be events around the city with a bike parade on Wednesday afternoon from Clontarf to St Anne's Park in Raheny.
Danish and Dutch experts are set to highlight best practices from both countries, and offer concrete steps for others to emulate their success in promoting cycling.
Local cycling activists say that Dublin is far behind other capital cities and far from safe for cyclists.
Dublin City Council Chief Executive Owen Keegan has pointed to the progress being made on this front.
"Contracts will be awarded on three major cycleway projects in the city centre this year; the Clontarf to City Centre cycleway, the Fitzwilliam Street cycleway and the Royal Canal Way project; while design work is ongoing on the Dodder greenway, Clonskeagh to city centre, and the remaining sections of the Sutton to Sandycove route," he said.
"With the Liffey cycle route now out for public consultation, all of these projects represent an important and exciting future for the city", Mr Keegan added.
Those attending the conference will get a chance to experience cycling in Dublin for themselves with bikes available at the conference centre and technical tours to the Grand Canal cycle route and the cycle parking facility in Drury Street.
While side events have already started the main conference opens tomorrow and runs until the afternoon of 28 June.