Luxembourg is challenging a European Union order to recover about €250m in back taxes from Amazon, saying it disagreed with EU state aid regulators' decision that the US online retailer had received special treatment.
The appeal to the Luxembourg-based General Court, Europe's second-highest, comes two months after the European Commission accused the Grand Duchy of giving illegal tax benefits to Amazon and sparing the company from paying taxes on almost three quarters of its profits.
"Luxembourg believes that the Commission has not established the existence of a selective advantage," its finance ministry said in a statement.
"Furthermore, Luxembourg does not share the Commission's analysis with regard to transfer pricing," the ministry added.
Amazon employs 1,500 people in Luxembourg, making it one of the biggest employers in the country of half a million.
Luxembourg has also appealed against a 2015 ruling ordering it to recover up to €30m in back taxes from Italian carmaker Fiat.