Gaza's civil defence agency said that Israeli forces had killed at least 46 people, including 36 in Gaza City, which Israel's Defence Minister has told residents to flee.
Mahmud Bassal, a spokesman for the rescue agency operating under Hamas authority, said several deaths resulted from attacks in the north of the territory, to the west of Gaza City.
Drone strikes also killed two people in Al-Zawayda and two people at a camp in Nuseirat, both in central Gaza, Mr Bassal added.
Two aid seekers were killed by Israeli gunfire southwest of Khan Younis in southern Gaza, he said.
The Israeli army, which has pursued an offensive against Gaza City since 16 September, said it had struck "a Hamas terrorist in the northern Gaza Strip".
Media restrictions in the territory and difficulties in accessing many areas mean AFP is unable to independently verify the tolls and details provided by the civil defence or the Israeli military.
The strikes come as Defence Minister Israel Katz warned the military was tightening its encirclement of Gaza City, telling residents to flee south, as Hamas weighs US President Donald Trump's plan to end nearly two years of war in the Palestinian territory.
"This is the last opportunity for Gaza residents who wish to do so to move south and leave Hamas operatives isolated in Gaza City," Mr Katz said in a statement shared with Israeli media.
"Those who remain... will be considered terrorists and terrorist supporters."
The war was triggered by the 7 October, 2023, Hamas attack on Israel that resulted in the deaths of 1,219 Israelis, mostly civilians, according to an AFP tally based on Israeli figures.
Israel's retaliatory offensive has killed at least 66,148 Palestinians, according to health ministry figures in the Hamas-run territory that the United Nations considers reliable.
Their data does not distinguish between civilians and combatants, but indicates that more than half of the dead are women and children.
As Israeli intensifies its offensive on Gaza City, the Red Cross has said it had been forced to temporarily suspend its activities there, warning that "tens of thousands... face harrowing humanitarian conditions".
"The intensification of military operations in Gaza City has forced the International Committee of the Red Cross (ICRC) to temporarily suspend operations at its Gaza City office and relocate staff to ICRC offices in southern Gaza to ensure staff safety and operational continuity," it said in a statement.
The development comes as Hamas' review of US President Donald Trump's Gaza plan stretched into a third day, a source close to the militant group said.
Mr Trump yesterday gave Hamas "three or four days" to respond to the plan he outlined this week with Israeli Prime Minister Benjamin Netanyahu, who has backed the proposal to end Israel's almost two-year war with the Palestinian militant group.
"Accepting the plan is a disaster, rejecting it is another, there are only bitter choices here, but the plan is a Netanyahu plan articulated by Trump," a Palestinian official, familiar with Hamas' deliberations with other factions, told Reuters.
"Hamas is keen to end the war and end the genocide and it will respond in the way that serves the higher interests of the Palestinian people," he said, without elaborating.
Israeli planes and tanks pounded residential neighbourhoods throughout the night, residents in Gaza City said. Local health authorities said that at least 35 people across Gaza had been killed by the military, most of them in Gaza City.
A strike on the old city in northwestern Gaza City killed seven people, while six people sheltering in a school in another part of the city were killed in a separate strike, medics said.
Meanwhile, the Israeli military issued new orders for people to leave for the south and said it would no longer allow those to return to the north, as Gaza City came under heavy bombing.
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The Israeli military also said that starting today it would no longer allow people to use a coastal road to move from the south to communities in the north.
It would remain open for those fleeing south, it said. Witnesses said Israeli tanks began moving towards the coastal road coming from the east, but were not yet there.
In recent weeks, few people have moved from the south to the north as the military has intensified its siege on Gaza City. However, the decision will put pressure on those who are yet to leave Gaza City and also prevent hundreds of thousands of residents who have fled south from returning to their homes, likely deepening fears in Gaza of permanent displacement.
The military took similar measures in the early months of the war, completely separating north and south, before later easing those measures in January during a temporary ceasefire.
Two more Palestinians, including a child, have died of malnutrition and starvation in Gaza in the past 24 hours, the territory's health ministry said, raising deaths from such causes to at least 455 people, including 151 children, since the war started.
Gaza City and surrounding areas are suffering from famine that will likely spread, afflicting more than half a million Palestinians, according to an August report by the IPC global hunger monitor.
Israel, which blocked all food from entering Gaza for almost three months this year, eased restrictions in July by allowing in more aid.
The UN says far more aid is needed and says it is unable to reliably distribute supplies in Gaza, blaming Israeli military restrictions on movements and a breakdown of law and order.
Israel says there is no quantitative limit on food aid entering Gaza and accuses Hamas of stealing aid, accusations the Palestinian militant group denies.
