Taoiseach Simon Harris said the "world is standing at the precipice of a horrific moment" and called for an urgent review of the EU-Israel Association Agreement amid, what he said, is "growing evidence of a prolonged humanitarian catastrophe" in Gaza.
Speaking at the Tullamore Show in Co Offaly, Mr Harris said the new round of Gaza ceasefire talks "have to be talks that have results".
Egypt, the United States and Qatar have scheduled the negotiations for Thursday, as fears grow of a broader conflict involving Iran and Hezbollah.
Mr Harris said: "There is an onus on every single country and every single bloc, including the European Union, to use every lever at its disposal to bring a real focus to those talks and to make sure that they do result in a cessation of violence.
"These can't just be talks about talks or another round of talks. They have to be talks that result in the violence stopping, that result in the war ending.
"I believe we have to look at all of the levers at our disposal to do that. I have consistently said, and I reiterate today, that there is an EU-Israel Association agreement in place. That association agreement contains human rights clauses."
The Taoiseach said he cannot comprehend how some member states in the European Union seem to think those human rights clauses are redundant.
"I just can't get my head around it.
"They are there for a reason and I expect them to be implemented and enforced, and, I reiterate my call for that today."
Mr Harris's request for a review of the agreement follows a call earlier this year by then taoiseach Leo Varadkar and Spanish Prime Minister Pedro Sánchez.
Mr Varadkar and Mr Sánchez wrote to European Commission President Ursula von der Leyen, calling for a review of whether Israel is compliant with its human rights obligations under the EU-Israel Association Agreement.
In an earlier statement, the Taoiseach said the weekend had "brought the grisly milestone of 40,000 deaths in Gaza a step closer" after the territory's health officials said an Israeli airstrike on a school killed around 100 people.
He described the attack on the al-Taba’een school as "gut-wrenching", adding that "490 of Gaza’s schools have been bombed or damaged since the start of the war".
"Ireland condemns outright such awful and wholesale loss of civilian life," he said.
The Taoiseach said he is "particularly disturbed" by reports from the United Nations that "the number of aid deliveries reaching Gaza has halved to fewer than 80 trucks a day in June and July".
He said that the UN Office for the Coordination of Humanitarian Affairs has reported that "only 24 of 67 planned aid missions to northern Gaza this month have been facilitated by Israel".
Mr Harris noted that more than 80% of Gaza is under evacuation orders, and Palestinians are seeking shelter in schools which are being attacked.
He said: "We have all been horrified by the many undoubted war crimes that have been committed in Gaza.
"There can be no impunity.
"Those responsible must be held to account."
The Taoiseach described the world as "standing at the precipice of a horrific moment and yet all levers to bring an end to the violence are not being used".
He said that "all the legally binding orders" of the International Court of Justice must also be "implemented in full".
"Too many innocent lives have been lost in ten months," he said.
Ireland, he said, calls for "an immediate ceasefire, the unconditional release of Israeli hostages and for aid trucks to flow unimpeded".
Additional reporting: Gail Conway
