Mater Private Healthcare has announced plans to reduce its workforce by almost 10%.
The organisation is seeking 95 voluntary redundancies as part of an overall cost reduction programme.
Mater Private Healthcare currently employs around 980 people in Dublin, Cork, Limerick, Drogheda, and Navan.
In a statement the company said the reduction in the number of people with health insurance has negatively impacted the sector.
"The recent announcement that tax relief on private health insurance has been reduced may cause further erosion," it added.
The company said it had made efforts to reduce its cost base but, "regretfully, Mater Private has no choice but to reduce headcount by around 95, which it will seek to do by way of a voluntary redundancy programme."
It described the move as "unavoidable" to safeguard future jobs and maintain patient care.