Venezuela is to expel three US diplomats, accusing them of meeting with opposition leaders and encouraging "acts of sabotage".
Venezuelan President Nicolas Maduro said Venezuelan authorities had for months followed the three, who have now been given 48 hours to leave the country.
It is the latest of several public disputes between Mr Maduro and the US since he won an April election following the death of his predecessor Hugo Chavez.
"We detected a group of US embassy officials dedicated to meeting the far-right and to financing and encouraging acts of sabotage against the electrical system and Venezuela's economy," the president said in a televised speech.
"I have the proof here in my hands," Mr Maduro added.
"Yankees go home! Get out of Venezuela! Get out of here! I don’t care what actions the government of Barack Obama takes."
The US Embassy had no comment or confirmation regarding the expulsions.
Venezuela is to expel Kelly Keiderling, who as US Chargé d'Affaires is the senior US diplomat in Venezuela because the US has no ambassador to the country.
According to a US Embassy website, she has been assigned to Caracas since July 2011 as deputy chief of mission and was temporarily serving as the Chargé d'Affaires.
Venezuela identified the other two diplomats as Elizabeth Hunderland and David Mutt.