Around 60 members members of the Irish Nurses and Midwives Organisation and SIPTU are staging a lunchtime protest outside the Mater Private Hospital in Dublin in opposition to pay cuts.
Notice has been served on the hospital for a full withdrawal of labour by members of the INMO and SIPTU from Monday 1 February.
The unions say the action is being taken as a response to pay cuts of between 5% and 7.5% in basic pay and a 5% cut in allowances.
The hospital has said there is increased competition in the market and the VHI, its biggest source of revenue, has cut payments to private hospitals.