Andy Murray's Wimbledon comeback ended in the third round with a straight-sets defeat by Denis Shapovalov.
By winning back-to-back matches at a grand slam for the first time since his hip problems started in 2017, the two-time Wimbledon champion had Centre Court dreaming of home glories once again.
But 10th seed Shapovalov was a step up from Nikoloz Basilashvili and Oscar Otte, and Murray was comfortably beaten 6-4 6-2 6-2 to complete a miserable day for British players
Fifth seed Andrey Rublev needed almost three hours to get the better of Fabio Fognini and reach the fourth round.
The result made it two Russian men already in the last 16 at the All England Club, with the chance of a third if Daniil Medvedev can triumph over Marin Cilic on Saturday.
Rublev had not made it past round two in any of his previous appearances at the Championships but a 6-3 5-7 6-4 6-2 victory extended the 23-year-old's best-ever run at SW19.
Karen Khachanov was the other Russian to progress from round three on Friday, and in fine fashion with a 6-3 6-4 6-4 success over Frances Tiafoe.
Maryland-born Tiafoe had made headlines with a first-round win over Stefanos Tsitsipas but he had little answer to the big-serving 25th seed.
Khachanov sent down 13 aces and offered up no break points to progress into the second week like he achieved back in 2018.
Roberto Bautista Agut showed his pedigree on grass with a hard-fought 7-5 6-1 7-6 (4) victory against Dominik Koepfer.
A surprise semi-finalist at the 2019 tournament, the Spaniard made it into week two for a fourth time from six appearances in the main draw.
Bautista Agut only registered 18 unforced errors and the 33-year-old will face the winner of the Friday night Centre Court battle between Andy Murray and 10th seed Denis Shapovalov.
Chilean Cristian Garin was the other player to book his place in the last 16 during the afternoon.
A 6-4 6-3 4-6 6-4 triumph over Pedro Martinez saw the 25-year-old extend his best-ever run at the All England Club.
Before this year's competition, Garin had never won a match at Wimbledon but could now be set for a date on the main stage next week with defending champion Djokovic if the Serbian can get the better of American Denis Kudla.
Marton Fucsovics has knocked out ninth seed Diego Schwartzman 6-3 6-3 6-7 (6) 6-4 in the third round at Wimbledon.
In a match lasting three hours and 19 minutes, it was the Hungarian who outlasted his opponent to continue his best-ever run at the All England Club.
Fucsovics made 47 unforced errors but also hit 40 winners to set up a last-16 meeting with Andrey Rublev.
It will be the fifth match between the pair during the last 12 months, not including a walkover given to Rublev at the Qatar Open in March. The fifth seed at the Championships beat Fucsovics in the fourth round at Roland Garros in 2020.
Dan Evans' Wimbledon third-round hoodoo continued after he lost to upcoming American Sebastian Korda on Centre Court.
The British number one has never made it to the second week at his home grand slam and falls at this stage for the third time after a 6-3 3-6 6-3 6-4 loss.
Evans, who also went out in the third round in 2016 and 2019, will be frustrated by this defeat as he was a break up in the fourth set, but lost four games in a row, missing the chance to send it to a decider.