Defending champion Garbine Muguruza has been beaten 5-7 6-2 6-1 by Belgium's Alison van Uytvanck in the second round of Wimbledon on Thursday.
The Spaniard suffered the earliest exit of a defending champion at the All England Club since Steffi Graf was beaten in the first round in 1994.
Van Uytvanck played the match of her life to dump Muguruza out.
Ranked 47 in the world, she put on a brilliant performance and won 5-7 6-2 6-2 in the All England Club twilight.
Muguruza was strangely out of sorts but could not cope with Van Uytvanck's tremendous hitting.
The Spaniard becomes the sixth top-10 seed to lose in the opening two rounds of this year's tournament.
Being scheduled last on Court Two perhaps left her vulnerable to a surprise, after a long day of waiting to play. But an upset looked unlikely after she got the better of a first set which had five break of serves.
Van Uytvanck, who has only got past the second round at a grand slam once before, began to find her range midway through the second set and levelled the match with a run of four successive games.
That hot streak continued as she hit Muguruza off the court and ended her trophy defence in remarkable fashion.
Angelique Kerber admitted to being caught cold as she had a battle to get past Claire Liu in three sets to progress to the third round.
The 2016 finalist was staring a defeat in the face after losing the first set, but came back to win 3-6 6-2 6-4.
Liu has pedigree as she is the 2017 junior champion and Kerber concedes she did not know too much about her 18-year-old opponent.
She said: "I didn't know a lot about her. This is always tough to play against someone you actually don't know, and also don't really know how she is playing.
"It was a quite tricky match. I was not playing my best today. I was not feeling my rhythm from the beginning. She played well. She had nothing to lose.
"For me it was just a match where I won. I'm just happy about that I won it at the end."
Kerber will next meet Naomi Osaka after the Japanese beat British favourite Katie Boulter 6-3 6-4.
And Osaka is expecting a tough outing against the German.
She said: "I think she does a lot of things good. She runs really well. She returns really well. I can't really say there's anything she does bad.
"She was number one in the world and won two grand slams, so for sure she's going to be a very hard opponent to play against."
Ashleigh Barty [above left] came out on top in a battle of Wimbledon junior champions with Eugenie Bouchard.
Australian Barty took the girls' title in 2011, a year before Bouchard, and was a 6-4 7-5 winner in their second-round contest on Court Three.
Bouchard, who also reached the women's Wimbledon final in 2014, had the chance to serve for the second set against Barty but lost four games in a row to sink in straight sets.
Barty's compatriot Daria Gavrilova won the all-Australian contest with Sam Stosur 6-4 6-1 while Barbora Strycova beat Lesia Tsurenko 6-1 6-4 in matches that were held over from Wednesday night.
Briton Johanna Konta was beaten in straights by world number 33 Dominika Cibulkova, losing 6-3 6-4 in 78 minutes.
"It feels great, it was a tough draw. I can only be happy with my performance, it was a great match," Slovakian Cibulkova said.
"It wasn't so difficult for me, I have experienced it many times -- the crowd were really nice to me. I was really happy to perform such good tennis on Centre Court."
Konta's defeat means she has not reached the third round at a major since last year's Wimbledon.
There were also wins for 14th seed Daria Kasatkina, Carla Suarez Navarro and Katerina Siniakova.
