Spain's Carlos Moya (shoulder) and Argentinian pair Juan Monaco (back) and Juan Ignacio Chela (shoulder) have withdrawn from Wimbledon citing injury.
They have been replaced by Martin Vassallo Arguello of Argentina, Croatia's Roko Karanusic and Argentina's Brian Dabul.
Moya, the 1998 French Open champion, has never enjoyed much success at the grasscourt slam with his best run coming in 2004 when he made the last 16.
Last year he was beaten in the first round by Tim Henman while he did not play in 2006 and 2005.
Japan's Akiko Morigami has withdrawn from the women's singles with a knee injury to be replaced by Renato Voracova of the Czech Republic.
