Venus Williams has pulled out of next week's WTA tournament in Charleston, increasing speculation about her health ahead of next month's French Open.
The American world number six and six-times grand slam champion also withdrew from this week's Amelia Island event for unspecified medical reasons.
'I regret that I will not be participating in the Family Circle Cup,' Williams was quoted as saying in US newspapers on Thursday.
The former world number one still intends to play in the Italian Open starting on 12 May.
'I look forward to returning to competitive play in Rome,' she said.
The French Open begins in Paris on 26 May.