Grimsby's reward for toppling Manchester United in the Carabao Cup is a third-round trip to crisis club Sheffield Wednesday.
The League Two Mariners pulled off one of the biggest shocks in the competition's history by beating the Premier League giants following a mammoth penalty shootout and will now travel to Hillsborough with a realistic shot of making the fourth round.
Liverpool will host Southampton and there is also a home tie for defending champions Newcastle as they welcome League One Bradford.
Arsenal, Chelsea, Tottenham and Manchester City will all also face third-tier opposition.
The Gunners visit Port Vale, Enzo Maresca's side head to Lincoln, Spurs host Doncaster, while City begin their quest to win the League Cup for the first time since 2021 with a trip along the M62 to face Huddersfield.
There are all-Premier League clashes as Brentford host Aston Villa, who met at the weekend in the league, and Wolves entertain Everton.
Nottingham Forest visit Championship side Swansea while Crystal Palace host Millwall in a repeat of last season's fiercely-contested FA Cup tie, which saw Jean-Philippe Mateta suffer a serious head injury.
Burnley host Cardiff, Brighton travel to League One Barnsley, Fulham host Cambridge, Wrexham welcome Reading and Wigan play Wycombe.
Ties will be played in the weeks commencing 15/22 September.
Carabao Cup third-round draw
Port Vale v Arsenal
Swansea v Nottingham Forest
Lincoln City v Chelsea
Tottenham v Doncaster
Brentford v Aston Villa
Huddersfield Town v Manchester City
Liverpool v Southampton
Newcastle v Bradford City
Sheffield Wednesday v Grimsby Town
Wolves v Everton
Crystal Palace v Millwall
Burnley v Cardiff City
Wrexham v Reading
Wigan v Wycombe
Barnsley v Brighton
Fulham v Cambridge
