Liverpool midfielder Ryan Gravenberch has been ruled out for at least the next two games with the ankle injury sustained in Sunday's Carabao Cup final victory over Chelsea.
The Dutchman was carried off on a stretcher after a tackle by Moises Caicedo and his absence will add to an injury list which already numbered 11 first-teamers before the weekend.
Fellow midfielder Wataru Endo left Wembley on crutches wearing a protective boot and he is also doubtful for the FA Cup fifth-round visit of Southampton.
Liverpool manager Jurgen Klopp said: "What I know is that Ryan is not available, along with all the others not available.
"It's a ligament injury, could've been much worse but bad enough for being ruled out of this game and next game.
"Wataru got a proper knock so we have to see as well. I wouldn't say it's much improved from Sunday.
"We need miracles with a few players but it is touch and go with a lot of players who were not available for the final. Darwin (Nunez), Mo (Salah), Dom (Szoboszlai), we have to see what they can do today."
Meanwhile Harvey Elliott accepts he will have to continue to push himself "to the max" as injuries threaten to derail the club's bid for an unprecedented quadruple.
The 20-year-old played the full 120 minutes of Sunday's win just four days after playing the entirety of the 4-1 victory against Luton, in which he scored a 90th-minute goal and had to be hauled off the turf at the end of the game.
His previous four appearances had all been as a substitute and amounted to just 153 minutes, but with 12 first team players unavailable through injury, fringe and academy players are now finding they are having to play more significant roles.
That is likely to be the case against Sky Bet Championship high-fliers Southampton in the fifth round of the FA Cup at Anfield on Wednesday - the third of four games in 15 days.
"Digging in deep - it came off the back of 90 minutes in midweek - is what you live for. This is why you're a footballer," said Elliott, who collapsed to the ground in exhaustion as the rest of the team ran to celebrate Virgil van Dijk's 118th-minute goal at Wembley.
"You need to push yourself to the max in order to get results and we did that.
"To come away with a victory is massive but we need to put it behind us, make sure we are recovered and focused on Wednesday.
"It will be another big test against Southampton as they are doing well this season and are going to cause us problems.
"I can't wait for another game."
