Manchester United midfielder Donny van de Beek has been forced to pull out of the Netherlands squad for Euro 2020 because of injury, the Dutch football association KNVB confirmed.
No details were given about the injury but the KNVB said examinations had concluded he would not be fit in time to play in the tournament, which kicks off at the weekend.
The Netherlands meet Ukraine in their opening Group C match in Amsterdam on Sunday.
The 24-year-old Van de Beek, who has 19 caps, had trained separately from the rest of the squad on Monday, along with Matthijs de Ligt. The defender has a groin injury and sat out Sunday's friendly win over Georgia in the last warm-up for the Dutch.
The KNVB said coach Frank de Boer would not be calling up a replacement for Van de Beek, even though the Dutch are entitled to replace him before their first game.
Chelsea goalkeeper Kepa Arrizabalaga has been added to Spain's training squad as they prepare for any potential fall-out from captain Sergio Busquets' positive Covid-19 test.
The squad's players will get a Covid-19 vaccine shot on Wednesday, the Spanish Sports Minister Jose Manuel Rodriguez Uribes confirmed.
After Busquets tested positive, the remaining 23 players went into isolation along with the coaching staff, meaning an Under-21 side led by coach age-group Luis de la Fuente will face Lithuania on Tuesday in what was supposed to be the nation's final warm-up game before the European Championships.
On Monday, Spain boss Luis Enrique called up Rodrigo Moreno (Leeds), Carlos Soler (Valencia), Brais Mendez (Celta Vigo), Pablo Fornals (West Ham) and Raul Albiol as potential cover should there be an outbreak.
Arrizabalaga and the five others will train in what Spain are calling a "parallel bubble" away from the main Euros squad with a view to them being added should the need occur.
The Spanish FA also confirmed that all the players and staff who are currently isolating tested negative, but will continue to train alone ahead of next Monday's Group E opener against Sweden in Seville.
Meanwhile, Poland forward Arkadiusz Milik will miss the tournament after withdrawing from the squad due to a knee injury, the Polish Football Association confirmed late on Monday.
The Olympique Marseille forward suffered a small meniscus tear in his left knee in the last Ligue 1 match of the season against FC Metz at the end of May. Since then he has not returned to normal training.
"The injury, with which the 27-year-old striker arrived at the Polish national team training camp in Opalenica, has eliminated the player from the tournament," the Polish FA said in a statement.
With Poland set to play their Group E opener against Slovakia on 14 June, Milik had been undergoing treatment over the last two weeks aimed at getting him back to full training.
However, he left Poland's training camp late on Monday to return to his club, which will take further steps to treat the injury.
The FA added it had decided not to call up a replacement for Milik, who has won 70 caps.
"The symptoms he experienced during training and the additional tests carried out afterwards confirmed that the injury still prevents him from full training and playing matches," the statement said.
"As the player himself admitted, despite the rehabilitation undertaken, he did not feel 100% ready to compete with full commitment in UEFA Euro 2020."
Milik's absence restricts coach Paulo Sousa's attacking options further having previously lost Hertha Berlin forward Krzysztof Piatek due to a broken ankle, and will place more of the scoring burden on Bayern Munich striker Robert Lewandowski.