Manchester City's Phil Foden was recalled to the England squad for the upcoming games with the Republic of Ireland, Belgium and Iceland - but there was no room for Manchester United forward Mason Greenwood.
The pair made their debuts in September's Group A2 opener against Iceland in Reykjavik, where they infuriated the FA by breaching UEFA's strict bio-bubble rules by meeting girls at the team hotel.
Southgate would have sent the pair home even if they had not broken coronavirus protocols, with the England manager leaving them out of October's matches as punishment.
Foden has since scored three times in six Premier League games and was recalled by Southgate as the Three Lions face Ireland in a Wembley friendly next Thursday, before heading to Belgium and welcoming Iceland to London as their Nations League group reaches a conclusion.
Greenwood, however, remains out in the cold with the 19-year-old once again missing the cut.
There seemed to be space for Greenwood as in-form Southampton striker Danny Ings is out injured, but Southgate opted against replacing him with either Greenwood or Newcastle's Callum Wilson.
Foden's club-mate Raheem Sterling was given an expected recall having missed the previous month's international break due to a small hamstring injury.
Other players who would have been pushing to be in contention but missed out include Aston Villa midfielder Ross Barkley and Manchester United's left-back Luke Shaw.
Shaw's Old Trafford team-mate Dean Henderson is the only uncapped player in Southgate's 29-man squad with fellow goalkeeper Jordan Pickford selected despite his ongoing inconsistent form for Everton.
Harry Maguire and Reece James are included after the pair were sent off as England lost 1-0 at home to Denmark last time out.
England boss Southgate hopes the matches will offer another opportunity for players to impress.
"Clearly managing the three games is a challenge, the friendly gives us another opportunity to find out about people, which we have done over the past few months," Southgate said at a press conference.
"Some of those players have really grasped the opportunity and are pushing for a place in the starting XI.
"We are trying to balance not too big a squad on matchdays, but equally some boys would not normally have the opportunity to be with us.
"It is a good experience for us, we will learn a lot about the players and there are some good games to look forward to."