Ireland manager Stephen Kenny must plan without captain Seamus Coleman ahead of this week's Euro 2020 play-off against Slovakia.
The Everton player picked up a hamstring injury in Saturday's 4-2 Premier League victory over Brighton, and was substituted early in the second half.
Everton manager Carlo Ancelotti suggested that the Irish captain picked up a "small injury", however, the FAI confirmed on Sunday evening that Coleman is ruled out of the must-win clash in Bratislava, while also adding that Cyrus Christie and Kevin Long had been added to the squad.
"[Seamus] seems to have a hamstring strain, which means that he will most likely be out," said Kenny earlier on Sunday.
"I knew when I saw the game, he was chasing Aaron Connolly. He is in brilliant form and was having an exceptional game yesterday."
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The Ireland manager said that he received a lot of phone calls on Saturday and he knew that they were not going to be the good type ahead of an international fixture.
Darragh Lenihan has definitely been ruled out of the trip as the Blackburn Rovers defender picked up an injury in training ahead of the weekend games.
As a result, Burnley defender, Long, who has been playing consistently in the heart of the Clarets' defence this season, has been called into the squad for Slovakia.
"Darragh Lenihan got injury in training, a knee in jury and missed Blackburn’s match at the weekend," confirmed Kenny.
"Kevin Long could consider himself unfortunate not to be call uped in the first place," added the manager.
"He is playing regularly in the Premier League and I'm delighted to have him in the squad."
Harry Arter is a also concern for the manager ahead of Tuesday's departure.
The Nottingham Forest midfielder came off injured in his game's 2-1 defeat to Bristol City on Saturday, and the manager confirmed that he is nursing a thigh injury.
Jack Byrne was also substituted in Shamrock Rovers' 4-0 victory over Sligo Rovers on Friday night, but the manager believes that the creative midfielder will be available to play.
"Harry Arter came off yesterday with slight thigh injury so we will see how that goes," said the manager.
"Jack Byrne, I believe he is ok."
Sunday's press conference began with the FAI thanking the Irish public for the support shown following the death of colleague Michael Hayes last week.
The FAI will issue a squad update later this evening, which will most likely confirm Coleman's absence from the squad.