Brentford missed the chance to move into the Championship's automatic-promotion places as their eight-match winning run came to an end with a 1-0 defeat at Stoke - a result which handed the title to Leeds.
Avoiding defeat would have seen the Bees replace West Brom in second spot ahead of Wednesday's final round of fixtures.
But Lee Gregory's close-range finish in the 38th minute, on the follow-up after David Raya parried an effort by Sam Clucas, proved the only goal of the game as Stoke's safety was sealed.
Bryan Mbeumo was denied by Potters goalkeeper Adam Davies prior to Gregory's finish, and Josh Da Silva shot wide of the hosts' goal just before the hour mark.
Substitute Emiliano Marcondes and Ethan Pinnock subsequently had strikes saved by Davies late on as Brentford continued to endure frustration.
Thomas Frank's men remain a point behind West Brom in third spot. They host rock-bottom Barnsley on Wednesday, while the Baggies take on QPR at the Hawthorns.
Leeds, whose promotion was already confirmed following Albion's 2-1 loss at Huddersfield on Friday, are now uncatchable at the top, sitting five points clear.
Meanwhile, the win for Michael O'Neill's Stoke in their last home match of the campaign left them eight points above the relegation zone in 17th.