Katie McCabe and Louise Quinn are gearing up for a trip to the Czech Republic after Arsenal were pitted against Slavia Prague in the Women's Champions League last-16, with Megan Campbell's Manchester City drawn against Atletico Madrid.
Scottish champions Glasgow City meanwhile face a two-legged tie against Danish club Brondby.
Women's Super League champions Arsenal, winners of the competition in 2007, will travel to the Czech capital for the first leg on October 16 or 17, before hosting the return match a fortnight later.
Manchester City and Glasgow will be at home first in their respective ties.
Arsenal cruised past Italian outfit Fiorentina in the last round, winning 6-0 on aggregate. City hammered FF Lugano 11-1 over two legs, while Ireland's Clare Shine scored one and made another in Glasgow's 4-1 defeat of FC Chertanovo Moscow that sealed their progression.
Holders Lyon, bidding for a record seventh title and fifth in a row, face Danish side Fortuna Hjorring, the 2003 runners-up.
The French club, who have England trio Lucy Bronze, Alex Greenwood and Izzy Christiansen in their ranks, will become the first club to play 100 matches in the competition during the first leg.
Two-time winners Wolfsburg will play Dutch side Twente, with Paris St Germain drawn against Icelandic club Breidablik.
Elsewhere, FC Minsk, the first Belarusian side to reach this stage, will play 2019 finalists Barcelona, while BIIK Kazygurt of Kazakhstan face Bayern Munich.
The final will be played on 24 May at Viola Park in Vienna.
Full draw: Glasgow City v Brondby, Barcelona v FC Minsk, BIIK Kazygurt v Bayern Munich, Lyon v Fortuna Hjorring, Paris St Germain v Breidablik, Wolfsburg v Twente, Slavia Prague v Arsenal, Manchester City v Atletico Madrid