Gareth Bale has been named in a 31-man squad for Wales' opening Euro 2020 qualifier despite missing Real Madrid's last game.
Bale turned his ankle in the closing moments of Real's Champions League defeat by Ajax and sat out the 4-1 LaLiga win at Real Valladolid on Sunday.
Rotherham midfielder Will Vaulks is called up for the first time as James Chester and Andy King miss out through injury.
Aston Villa defender Chester has not played since the end of January because of a knee problem, while Leicester midfielder King will not play again this season after damaging an ankle during a loan spell at Derby.
Ryan Giggs' squad meet Trinidad and Tobago in a Wrexham friendly on March 20 before starting their Euro 2020 campaign against Slovakia in Cardiff four days later.
Giggs also confirmed that Ashley Williams will remain as Wales captain during their Euro 2020 campaign.
The 34-year-old defender is on loan at Championship side Stoke from Everton.
"He will be captain - as long as he in the squad," Giggs said.
The Wales boss also explained the decision behind the team training at Manchester United's Carrington, as Colliers Park at Wrexham is not ready.
"It was a no-brainer in my head," Giggs added. "I want the best preparation for international football. The players were unanimously behind it."