Barcelona's board have rejected the idea of playing a La Liga game against Girona in Miami in January but have stated they are not against playing matches in the United States.
La Liga bosses are pushing for a fixture to be played in Miami and had suggested Girona's home match against Barcelona which is due to take place on 27 January.
Reports have claimed Manchester City's parent company, the City Football Group, who purchased a 44.3 per cent stake in Girona in August 2017, were keen on the game going ahead in Florida.
But despite La Liga president Javier Tebas telling Spanish radio station Onda Cero in September there was a 90 per cent chance the game would be between Girona and Barcelona, the idea has not been backed by the Nou Camp club's board.
A statement from the Barcelona board read: "The board of directors of FC Barcelona is not willing to play in Miami against Girona FC, after noting the lack of consensus on this proposal.
"FC Barcelona was and is willing to travel to Miami to play a league match, and accepted that the benefits be shared among all the First and Second Division clubs, following the same criteria of distribution of television rights, but consider that until an agreement is reached between all the agents involved, this project will not be able to prosper."
Tebas had said in September: "Girona-Barcelona will 90 per cent be played in the United States but the agreement is not about just one game, it's about a process which is much bigger."
La Liga is to earn €200million from a 15-year deal with the US entertainment company Relevent to play games in North America.
Tebas added: "There's no obligation for teams to play one game a year in the US, only the clubs who volunteer to go will go."
Tebas added that Girona fans would be offered free flights to the game which was due to be staged at the 65,000-capacity Hard Rock Stadium, home of NFL team the Miami Dolphins.