Tadhg Furlong, Cian Healy and Jamison Gibson-Park will all miss Ireland's crunch Six Nations game with France on Saturday but skipper Johnny Sexton is expected to be available.
Furlong remains sidelined with a calf issue while Healy and Gibson-Park, who were both late withdrawals from the opening-round win over Wales, are ruled out with hamstring injuries.
The Leinster trio will continue their rehab programmes with the Ireland medical team.
Sexton was due to complete his head injury assessment process on Monday, which would clear him to feature against the defending champions at the Aviva Stadium.
Hooker Rónan Kelleher is due to return to training this week after recovering from a hamstring complaint.
Connacht scrum-half Caolin Blade and Leinster loosehead Michael Milne have been called up by Ireland head coach Andy Farrell, who has also retained Roman Salanoa and Tom Stewart in his squad following their inclusions last week.
Farrell has suffered defeat in all three previous meetings with France while in charge of Ireland. Les Bleus began their title defence with a nervy win over Italy, while Ireland were convincing victors over Wales in Cardiff.
Backs: Bundee Aki, Caolin Blade, Ross Byrne, Craig Casey, Jack Crowley, Keith Earls, Jamison-Gibson Park, Mack Hansen, Hugo Keenan, Jordan Larmour, James Lowe, Stuart McCloskey, Conor Murray, Jimmy O'Brien, Jamie Osborne, Garry Ringrose, Johnny Sexton (capt), Jacob Stockdale.
Forwards: Ryan Baird, Finlay Bealham, Tadhg Beirne, Jack Conan, Gavin Coombes, Caelan Doris, Tadhg Furlong, Cian Healy, Iain Henderson, Rob Herring, Rónan Kelleher, Dave Kilcoyne, Joe McCarthy, Michael Milne, Peter O’Mahony, Tom O’Toole, Andrew Porter, Cian Prendergast, James Ryan, Roman Salanoa, Dan Sheehan, Tom Stewart, Josh van der Flier.
Watch live coverage of Ireland v France (Saturday 2.15pm) on RTÉ2 and RTÉ Player, follow our live blog on RTÉ.ie/Sport and the RTÉ News app, or listen to live commentary on RTÉ Radio 1.
Watch live coverage of England v Italy (Sunday, 3pm) on RTÉ2 and RTÉ Player.