England head coach Eddie Jones insists he hasn't given up on Mako Vunipola, Billy Vunipola, George Ford and Jamie George after all four were left out of 45-man training squad.
The quartet were among several established international players dropped by Jones, with Mako Vunipola and George having just toured with the British and Irish Lions.
Another Lion, Elliot Daly is also left out, although it's believed the versatile back is injured.
Ten of the 45-man group recently toured with the Lions in South Africa, including number eight Sam Simmonds, who last player for England in the summer of 2018.
Jones has also included eight uncapped players in his squad, with Louis Lynagh among those brought in.
The 20-year-old Harlequins wing is the son of Australia legend Michael Lynagh, and grew up in England after being born in Italy.
His Quins teammates Jack Kenningham and Sam Reilly also earn first call-ups, while Gloucester' Mark Atkinson and Ollie Sleightholme, Wasps hooker Gabriel Oghre, and Sale's Raffi Quirke and Bevan Rodd complete the uncapped group.
Eddie Jones has named a 45-player squad for a short training camp ??— England Rugby (@EnglandRugby) September 21, 2021
However, the big news remains the players who haven't been included, with the Vunipola brothers, George and Ford holding more than 250 England caps between them.
Jones, who has proved notoriously stubborn with his selections in recent years, insists they do still have a role to play in the future.
"This is an exciting squad made up of experienced players and young guys who did well in the summer and have earned their place again," Jones said.
"We have left out some experienced players but we're really clear that the door isn't closed to them, and we're looking forward to seeing them work hard to get back into contention.
"We have five campaigns now until the 2023 World Cup so each one counts, and this is a chance for this 45 to impress the new coaching staff."
The short training camp will bridge the gap until England's Autumn Series in November, where they face Tonga, Australia and South Africa.
Forwards: J Blamire (Newcastle Falcons), C Chick (Newcastle Falcons), L Cowan-Dickie (Exeter Chiefs), T Curry (Sale Sharks), T Davison (Newcastle Falcons), A Dombrandt (Harlequins), C Ewels (Bath Rugby), E Genge (Leicester Tigers), J Heyes (Leicester Tigers), J Hill (Exeter Chiefs), T Hill (Worcester Warriors), M Itoje (Saracens), J Kenningham (Harlequins), C Lawes (Northampton Saints), L Ludlam (Northampton Saints), L Ludlow (Gloucester Rugby), J Marler (Harlequins), G Martin (Leicester Tigers), B Obano (Bath Rugby), G Oghre (Wasps), S Riley (Harlequins), B Rodd (Sale Sharks), S Simmonds (Exeter Chiefs), K Sinckler (Bristol Bears), W Stuart (Bath Rugby), S Underhill (Bath Rugby).
Backs: M Atkinson (Gloucester Rugby), O Farrell (Saracens), G Furbank (Northampton Saints), O Lawrence (Worcester Warriors), L Lynagh (Harlequins), M Malins (Saracens), J Marchant (Harlequins), J May (Gloucester Rugby), R Quirke (Sale Sharks), A Radwan (Newcastle Falcons), H Randall (Bristol Bears), D Robson (Wasps), H Slade (Exeter Chiefs), O Sleightholme (Northampton Saints), M Smith (Harlequins), F Steward (Leicester Tigers), M Tuilagi (Sale Sharks), A Watson (Bath Rugby), B Youngs (Leicester Tigers).