Footage has emerged of a number of Barbarians players drinking alcohol in a London pub, including former Ireland winger Fergus McFadden.
The video that has been circulated on social media is from one of the two nights out that has resulted in the cancellation of Sunday's annual fixture at Twickenham for a breach of coronavirus protocols.
McFadden, former England captain Chris Robshaw, Sean Maitland, Jackson Wray, Joel Kpoku and Manu Vunipola are among the group who appear to be playing a drinking game at what is believed to be a pub in Mayfair.
An English Rugby Football Union investigation into the players' misconduct is ongoing, with a view to bringing disrepute charges against any wrongdoing.
Five players, including McFadden and Robshaw, have issued apologies for behaviour that is set to cost the RFU around £1m in lost TV and sponsor revenue.
"I want to front up and apologise profusely for my part in letting down @barbarians_fc," McFadden posted on Twitter.
"I greatly regret that the game will not be going ahead now. I am embarrassed and whole heartedly apologise to all involved. #sorry"
It comes at a time when the RFU are battling a financial crisis caused by the coronavirus pandemic that has resulted in 140 people being made redundant, amid a predicted £60m loss in revenue from autumn and Six Nations matches being played behind closed doors.
England's World Cup-winning coach Clive Woodward has described the players' conduct as "ridiculously stupid".
I want to front up and apologise profusely for my part in letting down @barbarians_fc. I greatly regret that the game will not be going ahead now. I am embarrassed and whole heartedly apologise to all involved. #sorry— Fergus McFadden (@fergmcfadden) October 23, 2020