Backs Ben Te'o and Mike Brown were excluded from the final week of an England training camp after being involved in an altercation during a team social event.
Former Leinster centre Te'o and full-back Brown were among four players cut from the training squad by coach Eddie Jones and neither was included in the matchday 23 for Sunday's World Cup warm-up Test against Wales at Twickenham.
Jones, who will finalise his 31-man squad for the World Cup on Monday, said that the players cut from the squad could still make it to Japan, where the World Cup kicks off on Sept. 20.
New Zealand born Te'o was previously in hot water with Jones and made to apologise to the squad after he and Billy Vunipola went on a late night drinking session after the Six Nations draw with Scotland last year.
Te'o left the Worcester Warriors at the end of last season.
Meanwhile Centre Henry Slade, flanker Sam Underhill and wing Ruaridh McConnochie have been ruled out of Sunday's warm-up against Wales by respective knee, toe and hip injuries.
Slade's place at 13 goes to Jonathan Joseph, Joe Cokanasiga comes in for McConnochie and Lewis Ludlum plugs the hole in the back row, where Tom Curry switches to openside.