Joe Marler has come out of international retirement to be named in England's Rugby World Cup training squad, while Danny Cipriani has also been included on the 35-man roster.
Harlequins prop Marler last featured for England against South Africa in June 2018 before retiring due to personal reasons, but he has now reversed that decision.
Gloucester's Willi Heinz has won the battle for inclusion at scrum-half, with Saracens' Ben Spencer also included, but Wasps' Dan Robson missing out.
England boss Eddie Jones said: "I saw Joe during the Barbarians' week and he indicated that he had a desire to come back.
"We have since had a couple of chats and we have given him the opportunity to do that. Now it is up to him to show us what he has got.
"We can't wait to have the full squad in place and to have players competing for those (final World Cup) 31 places.
"It is a process of building the team up, building the cohesion, the tactical understanding of the team and our adaptability. We will need to get those things right to win the World Cup, and that is our aim."
Gloucester fly-half Cipriani won the Premiership player of the year and players' player of the year awards in a sterling first campaign with the Cherry and Whites.
Now the 31-year-old has the chance to push for a final World Cup place.
Harlequins prop Kyle Sinckler has been included after a hamstring injury scare.
Harlequins full-back Mike Brown has missed out, while Saracens' Alex Lozowski has also been overlooked.
The continued absence of Dylan Hartley due to knee injuries leaves the former captain unlikely to make the autumn's World Cup.
Jones said: "World Cup selection is a four-year process. We have looked at the squad carefully, tried to build up a squad that has enough experience, attitude and energy to be winners and I think we have that.
"In the process, we have left out some players who can feel unlucky. For those guys who have missed out, the door is never closed but they have just got to be ready, as you never know when the opportunity comes."
England must submit their final 31-man World Cup squad on 2 September, and they start their campaign against Tonga in Sapporo on 22 September.
Jones added: "For the fans it is the start of the journey, so we want you to be with us, we are all in this together."
Dan Cole (Leicester Tigers, 85 caps), Luke Cowan-Dickie (Exeter Chiefs, 11 caps), Tom Curry (Sale Sharks, 10 caps), Charlie Ewels (Bath Rugby, 10 caps), Ellis Genge (Leicester Tigers, 9 caps), Jamie George (Saracens, 37 caps), Maro Itoje (Saracens, 27 caps, Joe Launchbury (Wasps, 58 caps), Courtney Lawes (Northampton Saints, 71 caps), Lewis Ludlam (Northampton Saints, uncapped), Joe Marler (Harlequins, 59 caps), Ben Moon (Exeter Chiefs, 8 caps), Brad Shields (Wasps, 8 caps), Kyle Sinckler (Harlequins, 22 caps), Jack Singleton (Saracens, uncapped), Sam Underhill (Bath Rugby 9 caps), Billy Vunipola (Saracens, 41 caps), Harry Williams (Exeter Chiefs, 17 caps), Mark Wilson (Sale Sharks, 13 caps)
Danny Cipriani (Gloucester Rugby, 16 caps), Joe Cokanasiga (Bath Rugby, 4 caps), Elliot Daly (Saracens, 30 caps), Owen Farrell (Saracens, 70 caps), George Ford (Leicester Tigers, 55 caps), Piers Francis (Northampton Saints, 4 caps), Willi Heinz (Gloucester Rugby, uncapped), Jonathan Joseph (Bath Rugby, 40 caps), Jonny May (Leicester Tigers, 45 caps), Ruaridh McConnochie (Bath Rugby, uncapped), Ben Spencer (Saracens, 3 caps), Henry Slade (Exeter Chiefs, 22 caps). Ben Te'o (unattached, 18 caps), Manu Tuilagi (Leicester Tigers, 32 caps), Anthony Watson (Bath Rugby, 33 caps), Ben Youngs (Leicester Tigers, 85 caps)
In camp for rehabilitation
George Kruis (Saracens, 32 caps), Jack Nowell (Exeter Chiefs, 33 caps), Mako Vunipola (Saracens, 53 caps)