Wasps flanker Thomas Young and Leicester wing Jonah Holmes will make their Six Nations debuts for Wales in Saturday's clash with Italy.
Young, who won two caps on Wales' 2017 summer tour against Tonga and Samoa, lines up in Rome alongside back-row colleagues Aaron Wainwright and Josh Navidi.
Centre Jonathan Davies captains his country for a first time - regular skipper Alun Wyn Jones is on the replacements' bench - as Wales head coach Warren Gatland makes 10 changes and one positional switch from the side that beat France last week.
There is also a Six Nations bow for Holmes, who made his Test debut during this season's autumn series, with scrum-half Aled Davies and flanker Wainwright handed first starts in the tournament.
Centre Owen Watkin, fly-half Dan Biggar, who replaces Gareth Anscombe, hooker Elliot Dee and lock Jake Ball also feature, with Navidi moving from blindside flanker to number eight.
Players that started the 24-19 victory over France who are not involved in Wales' match-day 23 this weekend include wing George North, centre Hadleigh Parkes and hooker Ken Owens as Gatland maximises a 31-man squad who have trained in Nice this week.
Young is the son of Wasps rugby director and former Wales prop Dai Young, and he has been among the Gallagher Premiership's most consistent performers this season.
He now has a glorious chance to show his Test credentials at the start of a World Cup year.
Victory for Wales at the Stadio Olimpico would make it 11 successive wins and equal their all-time unbeaten record set between 1907 and 1910.
They have also not lost to Italy for 12 years, while the Azzurri are without a win against any Six Nations team since 2015.
Gatland said: "We have made a number of changes this weekend, but have picked what we think is a very exciting team that still has huge experience throughout it.
"We have made a similar number of changes to what we did last year, but it is all about opportunity for these players. We have had a squad of 31 training together, replicating the Rugby World Cup, and it is important they get game time.
"It is a great opportunity for Jon (Davies) as captain. He has a huge amount of experience, is a great leader and it is a great honour for him to lead his country.
"Italy will be hurting after last weekend (they lost to Scotland), but they are a different proposition in Rome.
"They are an experienced team themselves and they will be looking to put things right on the pitch after their opening defeat."
Wales team to play Italy in Rome on Saturday: L Williams (Saracens); J Holmes (Leicester), J Davies (Scarlets, capt), O Watkin (Ospreys), J Adams (Worcester); D Biggar (Northampton), A Davies (Ospreys); N Smith (Ospreys), E Dee (Dragons), S Lee (Scarlets), J Ball (Scarlets), A Beard (Ospreys), A Wainwright (Dragons), T Young (Wasps), J Navidi (Cardiff Blues).
Replacements: R Elias (Scarlets), W Jones (Scarlets), D Lewis (Cardiff Blues), A W Jones (Ospreys), R Moriarty (Dragons), G Davies (Scarlets), G Anscombe (Cardiff Blues), H Amos (Dragons).
Italy coach Conor O'Shea, meanwhile, has made two changes for Saturday's clash.
Edoardo Padovani comes into the backline and Nicola Quaglio into the front row as Italy look to bounce back from their opening defeat to Scotland.
Padovani, who scored a try off the bench in the second half against Scotland, comes onto the right wing while Michele Campagnaro shifts back inside to his preferred position in the centre.
That means Tommaso Castello drops out of the matchday 23, while Quaglio replaces loosehead prop Andrea Lovotti, who has the flu.
Italy team v Wales: J Hayward (Benetton), E Padovani (Zebre), M Campagnaro (Wasps), L Morisi (Benetton), Angelo Esposito (Benetton), Tommaso Allan (Benetton), Guglielmo Palazzani (Zebre); Nicola Quaglio (Benetton) Leonardo Ghiraldini (Toulouse), Simone Ferrari (Benetton), David Sisi (Zebre), Dean Budd (Benetton), Sebastian Negri (Benetton), Abraham Jurgens Steyn (Benetton), Sergio Parisse (Stade Francais, capt)
Replacements: Luca Bigi, Cherif Traore, Tiziano Pasquali, Federico Ruzza, Marco Barbini, Edoardo Gori, Ian McKinley, Tommaso Benvenuti (all Benetton).
