England have dropped Mike Brown for the first time in the Eddie Jones era after he was omitted from a 25-man squad retained for Saturday's Test against South Africa.
Brown, 33, has been a regular under Jones, usually at full-back but playing on the wing with some success in the June tour of South Africa. However, he was not among the group of 25 who will remain in a training camp in Portugal.
His absence means Elliot Daly is likely to start at full-back, with Jonny May, Jack Nowell and Chris Ashton competing for the wing berths.
Also missing is Ben Morgan, who was recalled to the squad to cover the absence of Billy Vunipola. That means a likely start at number eight for Zach Mercer, one of three uncapped players in the group, which will be trimmed to 23 on Thursday morning.
Lock Courtney Lawes has been ruled out with a back injury but will stay in Portugal.