Kerry rider Oisin Murphy scored his first winner at this year's Royal Ascot as Dashing Willoughby landed a gamble in the Queen's Vase.
Murphy did not enjoy the best start to the week after failing a breath test before racing at Salisbury on Sunday, but he put that incident well behind him as he steered Andrew Balding's charge to victory.
Nayef Road set the pace in the 14-furlong heat with Barbados on his heels, while Murphy maintained a watching brief on the 6-1 winner and it was between those three from some way out.
Dashing Willoughby seized the initiative coming into the final two furlongs and despite drifting across the track, he held on by half a length, with Murphy punching the air as he passed the post.
Barbados snatched second for Aidan O'Brien, with the Mark Johnston-trained Nayef Road sticking to his guns well in third.
Earlier Frankie Dettori teamed up with Mark Johnston to win the Queen Mary Stakes, courtesy of Raffle Prize.
The 18-1 winner narrowly denied Kimari, who was bidding to give American trainer Wesley Ward another juvenile winner at the meeting.
It was nip and tuck inside the final furlong, but Dettori used all his experience to get up inside the final few strides and land his 61st Royal Ascot winner - going on to treat the crowd to a flying dismount.
In the colours of Sheikh Mohammed's son, Sheikh Hamdan bin Mohammed Al Maktoum, Dettori was greeted by his long-time former boss from the Godolphin days on his return.
Danny Tudhope chalked up his third winner at this year's Royal Ascot as Move Swiftly snatched the Duke of Cambridge Stakes on the line.
Tudhope enjoyed a red-letter day on Tuesday when winning the Queen Anne Stakes on Lord Glitters before adding the Wolferton Stakes with Addeybb, and he kept up the momentum with the William Haggas-trained Move Swiftly.
Sent off a 9-1 shot, Tudhope left it late in the Group Two heat, allowing Rawdaa and I Can Fly to play their hands before going for home in sight of the post.
Rawdaa, partnered by the treble-seeking Frankie Dettori, put up a brave fight, but Move Swiftly was just too strong, prevailing by a neck.