Dual Guineas winner Gleneagles and top-class filly Found will skip Epsom this weekend and will instead head directly to Royal Ascot.
It had been expected that Gleneagles would at least be left in the Investec Derby at Monday's confirmation stage, but owners Coolmore have decided to keep him to a mile in the St James's Palace Stakes at the Royal fixture.
Found, a Group One winner last season, missed the Guineas at Newmarket and was a narrow runner-up in the Irish version, but speculation had mounted that she could be supplemented to take on the colts.
She will now be prepared for the Coronation Stakes.
Coolmore confirmed that Aidan O'Brien is likely to be represented by Giovanni Canaletto, Hans Holbein and Kilimanjaro in the premier Classic at Epsom.
Ante-post favourite Golden Horn and Irish challenger Success Days have been supplemented at a cost of £75,000 each.
As well as Golden Horn, John Gosden will run Jack Hobbs, bought by Godolphin since his second-place finish in the Dante at York.
Andrew Balding's Elm Park and Criquette Head-Maarek's Epicuris are the only two previous Group One winners in the field.
The Dermot Weld-trained Zawraq has looked impressive in winning the only two starts of his career.
Storm The Stars, Moheet, Rocky Rider, Rogue Runner, Best Of Times and Carbon Dating complete the 15 remaining possible.
Gleneagles & Found run in St. James's Palace Stakes & Coronation Stakes respectively at @Ascot— Coolmore (@coolmorestud) June 1, 2015