The Baltimore Ravens clinched an NFL play-off spot following a 20-10 win over the Cleveland Browns.
Needing to win their final match of the regular season, whilst hoping the San Diego Chargers lost to the Kansas City Chiefs to grab the final wildcard spot in the AFC, Joe Flacco threw for 312 yards and two touchdowns, while Justin Forsett ran for 119 yards to seal the win.
The Chargers' inconsistent season ended in defeat as they were beaten by the Chiefs 19-7, who would have progressed had the Houston Texans and Ravens lost.
Chargers quarterback Philip Rivers threw two interceptions and was sacked seven times, four times by Justin Houston.
Back-up quarterback Chase Daniels was solid for the Chiefs as he threw for 157 yards without turning the ball over as he won his first career start.
JJ Watt made it 20.5 sacks for the year as he put Blake Bortles on his backside three times, but he will not be in the post-season despite the Houston Texans' 23-17 win over the Jacksonville Jaguars.
The New England Patriots had already wrapped up the top spot and home field advantage in the AFC and were beaten 17-9 by the Buffalo Bills.
The Indianapolis Colts went into their game against the Tennessee Titans already guaranteed the number four seed having already won the AFC South, but still won 27-10 against a side who will have the second pick in the draft.
Geno Smith threw a career-high 358 yards in what is likely to be head coach Rex Ryan's last game in charge of the New York Jets who beat the Miami Dolphins 34-24.
Number two seed Denver Broncos eased to a 47-14 win over the Oakland Raiders.
In the NFC, the Dallas Cowboys went into the post-season on a high after a 44-17 win over the Washington Redskins.
Tony Romo threw for 299 yards, while DeMarco Murray ran for 100 as the Redskins dropped to 4-12 for the season.
The Philadelphia Eagles backup quarterback Mark Sanchez threw two touchdowns in a 34-26 victory over the New York Giants in what could have been Tom Coughlin's final game as New York's coach.
The Tampa Bay Buccaneers ensured they will have the number one pick in next year's draft after they lost 23-20 to the New Orleans Saints, while the Chicago Bears lost 13-9 at the Minnesota Vikings.
The Carolina Panthers eased to the NFC South title after they thrashed the Atlanta Falcons 34-3 in their winner-takes-all contest.
The home side were dominant, especially on defense, with Falcons quarterback Matt Ryan picked twice and sacked six times as the Falcons failed to make the post-season.
The Green Bay Packers are the NFC's second seed and earned a bye after they beat divisional rivals the Detroit Lions 30-20 to take the NFC North - the Lions now face Dallas in the play-offs.
Star quarterback Aaron Rodgers seemed to pick up a calf injury when connecting with a touchdown pass but battled on to throw 226 yards. Matt Stafford threw for three touchdowns but it was not enough.
The Seattle Seahawks confirmed their place as the number one seed in the NFC conference following a 20-6 win over the St Louis Rams.
The San Francisco 49ers beat the play-off bound Arizona Cardinals 20-17.
San Francisco announced immediately after the game that they had agreed to part ways with coach Jim Harbaugh.
In the late game, Antonio Brown sealed the AFC North for Pittsburgh Steelers with a 63-yard touchdown in the 27-17 victory over the Cincinnati Bengals.