Shane Lowry and Seamus Power both missed the cut at the Safeway Open in California.
Open champion Lowry was unable to build on his steady start and shot a one-over 73 in the second round with two bogeys and just a solitary birdie in his round to finish on three under par.
Olympian Power improved on his poor opening round but his one-under 71 still left him a long way off the five-under cut mark as he ended up on two over par.
Sam Burns overcame an early double bogey to take a two-shot lead into the third round of the Napa event.
The American entered Friday's round trailing Scot Russell Knox by a stroke, but his attempt to chase Knox down got off to a disastrous start when he found the water on the 11th.
But after settling for a double bogey, Burns immediately clicked into gear - carding five birdies through the rest of the back nine.
He carried this momentum into the front nine, picking up another four birdies and an eagle - along with one bogey - to post a seven-under 65.
Two strokes back from Burns on 15 under is fellow American Harry Higgs, who shot a closing albatross for a second round of 62.
Knox finished Friday's round tied for third on 12 under, alongside Australian Cameron Percy and American DJ Trahan.
