Karolin Lampert carded a second successive 68 to climb to the top of the Andalucia Costa del Sol Open de Espana leaderboard early on Friday, before Christina Kim lit up the course with a brilliant hole in one.
Germany's Lampert enjoyed a fine day at Aloha Golf Club in Marbella, ending on eight-under-par and one stroke ahead of four other players.
Aditi Ashok, Marianne Skarpnord, Anne Van Dam and Nanna Koerstz Madsen, whose 66 was the low round of the day, are tied for second, with Olivia Cowan, Cheyenne Woods and Christina Kim a stroke further behind.
Leona Maguire meanwhile is three-over after following up yesterday's 73 with a 74. The Cavan 24-year-old will start as a full member of the LPGA tour when it resumes in January.
Kim went to the turn in 39, but picked up five shots in her last four holes, ending birdie, birdie, hole in one, birdie, to come back in 32 in a whirlwind finish.
Kim birdied holes 15 and 16, but the crowd went wild when she aced the 17th.
"Aces always feel great!" she said afterwards.
"The last hole in one I made in a tournament was in a Ladies European Tour event, in the Ladies European Masters at the Buckinghamshire, in 2013. Unfortunately, I couldn't see this one go in, it was 180 yards away and the way that the light was hitting us, we couldn’t exactly tell that it went in.
"People were clapping and cheering but it was a tentative high five with Aza Munoz and Anne Van Dam. It was a relief not to have to putt for one hole."